Composite

Part:BBa_K2151203

Designed by: Pinja Hartikainen, Liam Pollock, James Provan   Group: iGEM16_Glasgow   (2016-08-30)


p32-crtEBIY

This biobrick was created through standard biobrick assembly of K2151002(p32; a strong constitutive S. thermophilus promoter) K118014(RBS+crtE), K118006(RBS+crtB), K118005(RBS+crtI) and K118013(crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts converts colourless farnesyl pyrophosphate to orange beta-carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2032
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1568
    Illegal NgoMIV site found at 1698
    Illegal AgeI site found at 783
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None